Quantification ofCampylobacterspp. in chicken carcass rinse by real-time PCR
نویسندگان
چکیده
منابع مشابه
Cytokine mRNA quantification by real-time PCR.
Real-time PCR represents a new methodology that accurately quantifies nucleic acids. This has been made possible by the use of fluorogenic probes, which are presented in two forms, namely hydrolysis probes (also called TaqMan probes) and hybridisation probes. We decided to apply this methodology to cytokine mRNA quantification and this led us to the development of a protocol that provides an ea...
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For proper relative quantification by real-time PCR, compared samples should have similar PCR efficiencies. To test this prerequisite, we developed two quality tests: (i) adjustment of a test for kinetic outlier detection (KOD) to relative quantification; and (ii) comparison of the efficiency variance of test samples with the efficiency variance of samples with highly reproducible quantificatio...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2008
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2008.03943.x